what do you think?

[A TIN OF SALMON]

well this is what i rekon :wtf:

I believe that it is suited for preparation of continuous and discontinuous density gradients. For stabilization of membrane-bound particles, isolation of lymphocytes. Dialyzable material (including NaCl) max. 1 %. Better osmotic properties than sucrose, it preserves functional and morphological integrity and does not penetrate biological membranes. Ficoll® 400 is a neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution. Concentrations up to 50 % (w/v), covering a density range up to 1.2 g/ml, can be obtained without exceeding normal osmo larity. Suitable for producing density gradients for separation by centrifugation or sedimentation at unit gravity (especially valuable for the separation of cells which are sensitive to centrifugation and for separating cells of similar density but different size). It is also used as a hapten carrier, and in electrophoresis, hybridization and cryopreservation.


any help would'nt go a miss

[A TIN OF OATS]

I've been thinking about this a lot myself lately.

Reference:
1. Sambrock, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (B.15, 6.12)


Related Information and Products

Plant Cell Culture: Protoplast Preparation (Protocol)

Molecular Biology: Reagents for Centrifugal Separation - Overview

[A TIN OF ELV]

ill get me coat ;)

[A TIN OF SOUP]

Just move the dangleberry 3 clicks to the right...

Should be fine then!

[A TIN OF FEAR]

All statements are true in some sense, false in some sense, meaningless in some sense, true and false in some sense, true and meaningless in some sense, false and meaningless in some sense, and true and false and meaningless in some sense.

A public service clarification by the Sri Syadasti School of Spiritual Wisdom, Wilmette.

[A TIN OF SALMON]

well guys thx for clearing that up all oatsy i knew that would have troubling you too :;):

[atinof]

Go on salmon, where did you copy and apste that from ::D:

[A TIN OF FEAR]

this explains it all

The activity of many transcription factors is controlled through their subcellular localization. NFAT proteins, which play a key role in the immune response and in the development of cardiac and neuronal cells, represent a well known example of this type of regulation. In resting cells NFAT is present in the cytoplasm. Upon stimulation it becomes dephosphorylated at a large number of residues (? 10) by the phosphatase calcineurin and imported into the nucleus. Recent experiments suggest that the phospho- rylation state of NFAT controls the switching between two alternative conformations that are nuclear import and export conducive, respectively. We develop a mathematical model based on this mechanism to address the following questions: Why are multiple phosphorylation residues required? How do kinases and phosphatases control the cellular localization of NFAT? Does dephosphorylation at each single site specifies a distinct conformation or merely alters the probability of switching between two endpoint con- formations? Taking dephosphorylation and conformational transitions to be much faster than transport steps, we can reduce the differential equation system by a rapid equilibrium approximation. From its so- lution, the steady states, time constant and dynamics are analyzed under different conditions. The model shows that dephosphorylation of NFAT is required in both cytoplasm and nucleus for effective translocation. Multiple dephosphorylations create a steep threshold for transcriptional activation that may allow the filtering out of weak signals. The value of this threshold increases with the basal activity of NFAT kinases. Comparing our model with the alternative mechanism of progressive conformational changes, we find in both models identical predictions for the total nuclear/cytoplasmic distribution of NFAT. How- ever, the transcriptionally active form in the conformational switch mechanism shows a markedly steeper activation threshold

[king7]

ill get me coat ;)

TAXI!!!

[A TIN OF YODAN]

:wtf:
say again
:wtf:

[A TIN OF LEAD]

connect the Phoo Phoo bar to the giggle pin located just behinds Soups Dangleberry sorted :wtf: :wtf:

[A TIN OF PAINT]

You get fries with that?


Get SOme

Paint

[A TIN OF SNIPE]

between two alternative conformations that are nuclear import and export conducive, respectively.

so thats where it all started... the big bang :?: